BD CaliBRITE beads are designed for use with FACSComp or AutoCOMP software and the FACS family of flow cytometers (FACSCalibur, FACSort, FACScan. values for BD Calibrite beads. To edit, see page A Target file is also created for. HLA-B Although used by. BD FACSComp software, the file is not editable. Product Name: BD CALIBRITE BEADS. Synonyms: BD CALIBRITE BEADS; CALIBRITE BEADS. CAS: MF: MW: 0. EINECS: Mol File: Mol File. BD CALIBRITE .
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A thorough investigation of sucrose The suspensions are stable for a longer period of time beqds Bead Dilution Buffer. Optimize settings for your sample, as needed. The FSC threshold is adjusted to a level that minimizes background signal if any. After the instrument settings have been determined, BD Calibrite beads are used to evaluate instrument sensitivity.
BD Calibrite™ Beads
For information on use, refer to the appropriate instrument manual. The following list illustrates PMT light signal detection: Next, the software adjusts fluorescence compensation using a mixed-bead suspension containing equal amounts of the appropriate BD Calibrite beads. This allows cells to be distinguished from sample debris or background signal and for dimly stained cells to be distinguished from unstained cells.
Mix bead vials by gentle inversion or very gentle vortexing prior to use. Record PMT voltages and channel separations obtained for each parameter in a daily log sheet. FL1, FL2, and FL3 fluorescence sensitivity is determined by measuring the mean channel separation between the signal of the labeled beads and the unlabeled beads.
BD CALIBRITE BEADS
See examples in Optimization and Quality Control on page 4. BD Calibrite beads are for in vitro diagnostic use. Make sure to obtain a full drop of beads.
Beads used beyond their stability begin to show a decrease in separation between unlabeled and labeled populations, resulting in Sensitivity Test failure. Label two 12 x mm polystyrene tubes Tube A and Tube B. Because BD Calibrite beads simulate unstained cells and cells that have been stained labeled with fluorochrome-conjugated antibodies, the beads are used to adjust the instrument settings before cell beaes are run on the flow cytometer.
Calibritf instrument settings following two-color setup using a blood sample stained with any combination of monoclonal antibodies that identifies separate non-overlapping cell populations, such as FITClabeled and PE-labeled monoclonal antibodies.
Following PMT and compensation adjustment, the software performs a Sensitivity Test using the appropriate mixed-bead suspension. NOTE Over a period of time, the fluorescence separation might decrease. In some cases the software may not be able to automatically set up the instrument.
BD Calibrite™ Beads
Optimization following three- and four-color setup can vary depending on the application. Figure 1 through Figure 4 show examples of optimization for two- three- and four-color applications. Beads used beyond their stability begin to show a decrease in separation between unlabeled and labeled populations, possibly resulting in Sensitivity Test failure.
Notice populations with a lower FSC signal than lymphocytes debris, for example can be excluded by increasing the FSC threshold level. Gently mix the BD Calibrite bead vials, then add 1 drop of beads to each tube as indicated in the table below. UV Bead lab with graph. Do not use after the expiration date shown on the label.
The light scatter sensitivity is determined by the amount of channel separation between the mixed bead population and instrument background signal.
Refer to the information appropriate to the instrument setup you are performing. The beads are used to adjust instrument settings, set fluorescence compensation, and check instrument sensitivity. If you require any additional license to use this product and do not have one, return this material, unopened, to BD Biosciences, Qume Drive, San Jose, CAand any money paid for the material will be refunded.
The 2color kit contains three different types of BD Calibrite beads: Use the same staining method and run in parallel with the test samples. Weather and Climate for Educators. It might be necessary to adjust the FSC and SSC amplifiers so that all leucocyte populations are on scale, and to calivrite compensation and threshold settings see Figure 1.
Adjust fluorescence compensation using Tube B. Always refer to the appropriate application note or reagent IFU. The drop should be cloudy, indicating ebads beads are properly mixed.
BD Calibrite PerCP-Cy5.5 Beads
This product is licensed for sale only for in vitro diagnostics. Optimizing Scatter Figure 1 calbirite a lysed whole blood LWB sample from a normal donor before and after optimization. Adjustment is similar for PerCP-Cy5. Compensation adjustments for FL1, FL2, and FL3 correct for spectral overlap by shifting the labeled bead populations so they are aligned with the corresponding unlabeled bead populations.
It is not licensed for any other use.